Molecular Detection of Rifampicin and Isoniazid Resistance and Characterization of Mutations in Mycobacterium tuberculosis Complex using Line Probe Assay

Line probe assay for detection of rifampicin and isoniazid resistant tuberculosis in Pakistan.

OBJECTIVE To assess the efficacy of a line-probe assay delta (LiPA) as rapid diagnostic test for early detection of drug-resistant tuberculosis compared to conventional susceptibility methods in Pakistan. METHODS Resistance to rifampicin (RIF) and isoniazid (INH) in 108 smear-positive pulmonary tuberculosis samples was detected using a line-probe assay [GenoType MTBDRplus (Hain Lifescience, G...

Molecular characterization of mutation associated with rifampicin and isoniazid resistance in Mycobacterium tuberculosis isolates.

Nucleotide changes in catalase peroxidase (Kat G) gene and gene encoding the beta subunit of RNA polymerase (rpo B), responsible for isoniazid and rifampicin drug resistance were determined in the clinical isolates of Mycobacterium tuberculosis by PCR-RFLP, Line probe assay and DNA sequencing. PCR-RFLP test was performed by HapII cleavage of an amplified fragment of Kat G gene to detect the tra...

Molecular tests for rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis.

2. Molecular testing based on katG and rpoB enabled rapid detection of Mycobacterium tuberculosis resistance in 46.8% and 87.5% of patients infected with isoniazidand rifampicinresistant strains, respectively. The same number of patients infected with resistant strains with mutations in katG and rpoB were detected using the MTBDRplus line probe assay. The combined use of katG and inhA regulator...

Minor Contribution of inhA-15 Mutations to the Rapid Detection of Isoniazid Resistance in Mycobacterium Tuberculosis Isolates

Rapid Detection of Rifampicin and Isoniazid Resistant Mycobacterium tuberculosis Using Genotype MTBDRplus Assay in Nepal

Rapid line probe assay (LPA) can be a practical and rapid alternative to the slow conventional phenotypic drug susceptibility testing (DST) for detection of drug resistant tuberculosis (TB). The purpose of this study is to determine the diagnostic accuracy of Genotype MTBDRplus, LPA for TB, and compare its performance with conventional DST. A total of 54 culture samples were analyzed for DST us...